Bio-Rad Bio-Plex Pro™ Human Th17 Cytokine Assays - Manual (Page 10)

8
1. Plan Plate Layout
Prior to running the assay, determine the total number of wells in the
experiment using the plate layout template on page 34, or the Plate
Formatting tab in Bio-Plex Manager
™ . A suggested plate layout is shown
in Figure 2, with all conditions run in duplicate.
1. Assign standards to columns 1 and 2, with the highest
concentration in row A and the lowest concentration in row H.
2. Assign the blank to wells A3 and A4. The blank should consist of your
chosen standard diluent. Note that Bio-Plex Manager ™ automatically
subtracts the Blank (B) FI value from all other assay wells.
3. User-specified controls, as well as the quality controls supplied in
premixed Th17 cytokine kits, are assigned to wells in colums 3 and 4.
4. The remainder of the plate is available for samples.
5. Once the total number of wells is known, you can calculate the
required volumes of beads, detection antibody, and streptavidin-PE.
Use Tables 6, 9, and 11, respectively, or the Calculation Worksheet
on pages 35–36.
Fig. 2. Suggested plate layout. For detailed instructions on
plate formatting in Bio-Plex Manager, see Section 8. Legend
S Standards
B Blank
X Samples
C Controls