Bio-Rad Bio-Plex Pro™ Human Th17 Cytokine Assays - Manual (Page 24)

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9. After the streptavidin-PE incubation step, slowly remove and discard
the sealing tape.
10. Wash the wells three times with 100 µl of wash buffer according
to the wash method of choice.
11. To resuspend beads for plate reading, add 125 µl assay buffer to
each well. Cover the plate with a new sheet of sealing tape. Shake
at RT at 850 ±50 rpm for 30 sec and slowly remove the sealing
tape. Ensure that the plate cover has been removed before placing
the plate on the reader.
8. Read Plate
Bio-Plex Manager ™ software is recommended for all Bio-Plex Pro ™ assay
data acquisition and analysis. Instructions for Luminex xPONENT software
are also included. For instructions using other xMAP system software
packages, contact Bio-Rad Technical Support or your regional Bio-Rad
field applications specialist.
Prepare Protocol in Bio-Plex Manager Software v 6.0
and Higher
The protocol should be prepared in advance so that the plate is read as
soon as the experiment is complete.
A protocol file specifies the analytes in the assay, the plate wells to be
read, sample information, the values of standards and controls, and
instrument settings.
Bio-Plex Manager software versions 6.0 and higher contain protocols for
most Bio-Plex
® assays. Choose from available protocols or create a new
protocol. To create a new protocol, select File, then New from the main
menu. Locate and follow the steps under Protocol Settings.