Bio-Rad Bio-Plex Pro™ Human Th17 Cytokine Assays - Manual (Page 25)

23
1. Describe Protocol and enter information about the assay (optional).
2. Select Analytes and create a new panel. Visually confirm the selected
analytes and proceed to step 3.
a. Click the Add Panel button in the Select Analytes toolbar.
Enter a new panel name. Select Bio-Plex Pro Assay Magnetic
from the assay pull-down menu. If using Bio-Plex Manager version
5.0 or lower, select MagPlex from the assay pull-down menu.
b. Click the Add button. Enter the bead region number and name
for the first analyte. Click Add Continue to repeat for each analyte
in the assay. Refer to the bead regions in parentheses ( ) listed on
the peel-off label provided with the standards.
For reference, bead regions for human Th17 cytokine assays are
shown in Table 12.
c. Click the Add button when the last analyte has been added and
click OK to save the new panel.
d. Highlight analytes from the Available list (left) and move to the
Selected list (right) using the Add button. To move all analytes at
once, simply click the Add All button.
Analyte Bead Region Analyte Bead Region
IL-1 b 39 IL-23 43
lL-4 52 IL-25 55
lL-6 19 IL-31 62
IL-10 56 IL-33 46
IL-17A 76 IFN- g 21
IL-17F 44 sCD40L 29
IL-21 47 TNF- a 36
IL-22 18 IL-17A/F* 66
Table 12. Bead regions for human Th17 cytokine assays.
* Available only as a singleplex and in Express assay format.