Bio-Rad Bio-Plex Pro™ Human Th17 Cytokine Assays - Manual (Page 13)

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4. Prepare Standards and Controls
General Instructions
• It is essential to prepare standards and quality controls (if included)
exactly as described in this section. Incorrect preparation may lead to
low signal or variable measurements from plate to plate
• The product data sheet or peel-off sticker provided with the standards
lists the most concentrated point on the standard curve (S1). Enter this
information into Bio-Plex Manager
™ software as instructed in Section 8
Using the Quality Controls (optional)
Two-level quality controls (high and low) are included with premixed
Th17 cytokine panels and custom x-Plex kits. Their use is intended for
monitoring the day-to-day quality of assay results.
Selecting a Diluent for Standards and Controls
Refer to Table 4 for recommended diluents based on different sample types.
In order to meet the lot-specific control ranges provided on the product data
sheet, both the standards and controls should be reconstituted in Bio-Plex
®
standard diluent HB. If reconstituting in a different diluent, users will need to
establish/validate their own control ranges or acceptance criteria.
Table 4. Summary of recommended diluents for standards and controls.
Diluent for Standards
Sample Type and Controls* Add BSA
Serum and plasma Standard diluent HB None
Culture media, with serum Culture medium None
Culture media, serum-free Culture medium To 0.5% final**
Lavage, sputum, other fluids Sample diluent HB To 0.5% final**
Lysate Sample diluent HB To 0.5% final**
* If using diluents other than standard diluent HB, users must establish their own control ranges.
** At least 0.5% final BSA is recommended to stabilize analytes and reduce absorption to labware.