Bio-Rad Bio-Plex Pro™ Human Th17 Cytokine Assays User Manual
Page 26
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e. If some of the analytes need to be removed from the Selected
list, highlight them and select Remove. If desired, it is possible to
rename the panel by clicking on Rename Panel and entering a
new panel name.
3. Format the plate in Bio-Plex Manager
™
according to the plate layout
created in Section 1 (Plan Plate Layout). To modify the plate layout,
follow the steps below (see Figure 4).
a. Select the Plate Formatting tab.
b. Select the standards icon and drag the cursor over all
the wells that contain standards. Repeat this process for
Blanks , Controls , and Samples . Note that
Bio-Plex Manager
™
automatically subtracts the blank FI value
from all other assay wells.
S
B
X
C
Fig. 4. Plate formatting
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