Bio-Rad Bio-Plex Pro™ Human Th17 Cytokine Assays - Manual (Page 7)

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Item
Bio-Plex Pro Assays Quick Guide 1
Bio-Plex ® 200 system or Luminex system with HTF
Bio-Plex validation kit
Note: the validation kit should be run monthly to ensure
performance of fluidics and optics systems
Bio-Plex calibration kit
Note: the calibration kit should be run either daily or
whenever the instrument is to be used to standardize
fluorescence signal
Bio-Plex Pro wash station
For use with magnetic bead-based assays only
Bio-Plex Pro II wash station
For use with both polystyrene (nonmagnetic) and magnetic
bead-based assays
Bio-Plex handheld magnetic washer
For use with magnetic bead-based assays only
Bio-Plex Pro flat-bottom plates (40, 96-well plates)
For magnetic separation on the Bio-Plex Pro wash station
Microtiter plate shaker
IKA MTS 2/4 shaker for 2 or 4 microplates
or
Barnstead/Lab-Line Model 4625 plate
shaker (or equivalent capable of 300–1,100 rpm)
Bio-Rad Aurum ™ vacuum manifold
For vacuum filtration
BR-2000 vortexer
Reagent reservoirs, 25 ml
For capture beads and detection antibodies
Reagent reservoir, 50 ml (for reagents and buffers)
Pall Life Science Acrodisc: 25 mm PF syringe filter
(0.8/0.2 µm Supor membrane)
Filter plate, 1 x 96 with clear plastic lid and tray
Ordering Information
Item #10022581 (download at
www.bio-rad.com/bio-plex)
Bio-Rad catalog #171-000205
Bio-Rad catalog #171-203001
Bio-Rad catalog #171-203060
Bio-Rad catalog #300-34376
Bio-Rad catalog #300-34377
Bio-Rad catalog #170-20100
Bio-Rad catalog #171-025001
IKA catalog #320-8000
VWR catalog #57019-600
Bio-Rad catalog #732-6470
Bio-Rad catalog #166-0610
VistaLab catalog #3054-1002
or
VistaLab catalog #3054-1004
VistaLab catalog #3054-1006
Pall Life Sciences
catalog #4187
Bio-Rad catalog #171-304502
Other: 15 ml polypropylene tubes for reagent dilutions. Calibrated pipets (multichannel pipets
are optimal for this application) and pipet tips, sterile distilled water, aluminum foil, absorbent
paper towels, 1.5 or 2 ml microcentrifuge tubes, and standard flat-bottom microplate (for
calibrating vacuum manifold).
Table 2. Recommended materials.