Bio-Rad Bio-Plex Pro™ Human Th17 Cytokine Assays - Manual (Page 29)

27
Data Analysis
Quality Controls
If the quality controls were run in the assay plate, open the results (.rbx) file,
click on Report Table, and locate the control wells. Visually compare the
observed concentrations of the high and low controls in the Report Table
against the lot-specific control ranges shown in the product data sheet.
Expected control ranges are provided for reference and should be used
as general guidelines. Actual results may vary for some operators. If
the controls do not fall within the expected ranges, please refer to the
troubleshooting section for possible causes and solutions.
Removing Outliers
Outliers are identified as standard data points that do not meet accuracy
or precision requirements and should be considered invalid when
performing curve fitting. As such, they should be removed to generate a
more realistic and accurate standard curve. This may result in an extended
assay working range and allow quantitation of samples that might
otherwise be considered out of range (OOR).
In Bio-Plex Manager software version 6.0 and higher, outliers can be
automatically removed by selecting the Optimize button in the Standard
Curve window. In Bio-Plex Manager software 6.0 and earlier versions,
outliers also can be manually selected in the Report Table. Visit online Help
to learn more about the standard curve optimizer feature and how outliers
are determined.
Previous Versions of Bio-Plex Manager Software
For instructions on using previous versions of Bio-Plex manager software,
please contact Bio-Rad Technical Service.