ChemoMetec SP-100 - Manual (Page 39)

6 Troubleshooting
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6 Troubleshooting
Occasionally difficulties with the SemenView software may occur. In the following a
short description and suggested action is given.
6.1 Quality of the sample
Through the CM file image it is possible visually to assess the quality of the sample
preparation and thereby the reliability of the measurement by looking at the “starry
sky”. The measurement is reliable if the stars or white dots are distributed separately
and evenly over the image. Naturally, this rule only applies when a representative
sample has been drawn from the original Semen cell suspension.
Be aware of large objects in the image. These objects can be external material, such as
dust or a piece of hair. These objects are ignored in the image analysis so the obtained
result correctly reflects the number of individual cells in the sample. On the other hand,
it is important to be aware of these objects. If it is a cell aggregate or clumps, it may
indicate that the sample preparation has been sub optimal and the quality of the
estimate of cell concentration may be compromised.
6.2 Data not received
If SemenView does not receive data from the NucleoCounter SP-100, please verify that
the USB cable is connected correctly to both the NucleoCounter SP-100 and the
computer. Then look at the task bar on the computer to see if the NC Receiver is started.
Figure 40 NC Receiver icon on the Windows task bar
The NC Receiver must be started to receive data from the NucleoCounter SP-100. If the
NC Receiver is not open, quit and restart SemenView. NC Receiver should then appear
on the task bar.
Generally the NC Receiver has two states in the Info text field when it is started:
• Input open failure
• Input opened