Jenway 73 Series PC software - Manual (Page 47)

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Once the standards have
been measured click on
Show Curve to view the
quantitation curve. The curve
fit can be adjusted in this
screen. Click on Close
window to return to the
create curve screen.
Click on OK to save and
return to the measurement
mode.
6.3 CALIBRATION
Once the settings have been entered and if required a new quantitation curve has been created, the
zero calibration should be performed.
Insert the cuvette containing the blank sample into the sample chamber and close the instrument lid.
Click on the Zero button; this sets the instrument to zero absorbance and 100% transmittance.
Once a calibration is complete the
Read and Auto Log buttons are
activated.
If the settings menu is re-entered following the zero calibration it is possible to change the
wavelength. However a warning will be displayed to warn that critical settings are being changed after
the zero calibration has been performed and a quantitation curve has been created.