Leica Biosystems CM3050 S - Manual (Page 40)

40 Instructions for Use V 1.7 RevC - 08/2014
5.5 Daily operation of the instrument
• Select chamber temperature and, in instru-
ments with specimen cooling, specimen
temperature according to the type of speci-
men to be sectioned (see also chapter 6.3
‘Reference chart for temperature settings’).
• Do not continue with step 5.5.1 before the
actual chamber / (specimen) temperature
corresponds to the set value. This may take
several hours (e.g. when operating the in-
strument for the first time or when the in-
strument had been switched off).
Prior to operating the instrument,
please read and observe the following
safety instructions:
• ‘Integrated safety devices’
(Chapter 2.2) .
• ‘Handling microtome knives /
blades’ (Chapter 2.3.4)
• ‘Knife guard / Handwheel lock’
(Chapter 2.3.5)
• ’Motorized sectioning’
(Chapter 2.3.6)
• ‘Defrosting/ handling frozen tissue’
(Chapter 2.3.7)
• ‘Frozen instrument parts and acces
sories’ (Chapter 2.3.8)
• ‘Infectious / radioactive material’
(Chapter 2.3.9)
5.5.1 Freezing specimens onto specimen
discs with quick-freeze shelf and
stationary heat extractor
• Size the specimen.
• Apply sufficient amount of embedding me-
dium onto ambient temperature specimen
disc.
• Inside the cryochamber, place the specimen
onto the disc and orient.
• Place specimen disc with specimen into one
of the 10 openings in the quick-freeze shelf
(see below) and freeze.
The freezing process can be sped up further by
using the stationary heat extractor (1). Next to
the low temperature stabilizer (2) (‘Parking sta-
tion’), 3 specimen discs (3) can be placed within
its pivoting range.
• Place the heat extractor (1) directly onto the
specimen surface. After approx. 30 seconds
the specimen is completely frozen.
1 2
3
5. Operation