Leica Biosystems CM3050 S - Manual (Page 5)

5 Leica CM3050S – Cryostat
Table of Contents
4. Instrument Properties ..................................................................................................21
4.1 Overview ..............................................................................................................................................21
4.2 Technical Data ....................................................................................................................................23
5. Operation ........................................................................................................................25
5.1 Operating the instrument for the first time .....................................................................................25
5.2 Switching on/fuses .............................................................................................................................26
5.3 Control panel 1 ....................................................................................................................................27
5.3.1 Key functions in control panel 1 .......................................................................................................27
5.3.2 Display functions in control panel 1 ................................................................................................27
5.3.3 Display indications when switching on ..........................................................................................28
5.3.4 Menu functions: setting parameters of refrigeration, time and preset counter ......................29
5.3.5 Status display ......................................................................................................................................32
5.3.6 Indication of refrigeration state, section thickness setting and counters ................................33
5.4 Control panel 2 ....................................................................................................................................34
5.4.1 Selecting a sectioning mode / start and stop sectioning ............................................................35
5.4.2 Setting the sectioning window .........................................................................................................36
5.4.3 Selecting sectioning speed ..............................................................................................................37
5.4.4 Emergency stop ..................................................................................................................................37
5.4.5 Coarse feed keys ................................................................................................................................38
5.4.6 Trimming and sectioning functionsswitching between trimming and sectioning ...................39
5.4.7 Setting the section thickness ...........................................................................................................39
5.5 Daily operation of the instrument ....................................................................................................40
5.5.1 Freezing specimens onto specimen discs with quick-freeze shelf and
stationary heat extractor ...................................................................................................................40
5.5.2 Freezing specimens onto specimen discs with 90 ° prism
(Instruments w/specimen cooling only) .........................................................................................41
5.5.3 Optional accessories for freezing specimens onto specimen discs
and for removing frozen specimens ...............................................................................................41
5.5.4 Inserting specimen discs into the specimen head .......................................................................42
5.5.5 Specimen orientation .........................................................................................................................42
5.5.6 Trimming / sectioning ........................................................................................................................43
5.6 Finishing work .....................................................................................................................................44
5.6.1 Daily routine steps ..............................................................................................................................44
5.6.2 Switching off the instrument for a longer period of time .............................................................44
5.7 Defrosting ............................................................................................................................................45
5.7.1 Automatic chamber defrost cycle ...................................................................................................45
5.7.2 Manual chamber defrost cycle ........................................................................................................46
5.7.3 Defrosting the specimen head (instruments with specimen cooling only) ..............................46