Leica Biosystems CM3050 S - Manual (Page 51)

51 Leica CM3050 S – Cryostat
6. Troubleshooting, applications tips
Problem Causes Remedies
Sections tear. - Temperature too low for the tis-
sue to be sectioned.
- Knife blunt, dirty, dusty, frosted or
rusty.
- Upper edge of anti-roll plate dam-
aged.
- Hard particles in the tissue.
- Rear face of knife dirty.
- Select higher temperature
and wait for the new tem-
perature to be reached.
- Eliminate cause (--> see de-
tails on individual problems).
- Exchange anti-roll plate.
- If application permits, sec-
tion at lower plane.
- Clean.
Inconsistent or insufficient speci-
men feed.
- Microtome was not completely
dry when refrigeration was
switched on; ice build-up inside
the micrometer mechanism.
- Microtome defective.
- Remove microtome from
chamber and dry - possibly
with hairdryer (hairdryer
only to be used outside the
cryochamber!) or at ambient
temperature.
- Call Technical Service.
Specimen disc cannot be
removed.
- Specimen disc stuck to quick-
freeze shelf or specimen head
due to moisture on underside of
specimen disc.
- Apply strong alcohol to con-
tact point or heat the speci-
men head.
Cryostat inoperational. - Mains plug not properly con-
nected.
- Automatic cut-out has been trig-
gered.
- Check mains plug connection.
- Reconnect automatic cut-out
(switch instrument back on).
Sectioning motor inoperational. - Footswitch not connected.
- Automatic cut-out of sectioning
motor has been triggered.
- Motor defective.
- Connect footswitch.
- Reconnect automatic cut-out
(push inwards until it locks in
place).
- Call Technical Service.
No or insufficient refrigerating
performance.
- Stopper not placed properly into
drain in bottom of cryo-chamber.
- Compressor defective.
- Leak in cooling system.
- Close drain with stopper.
- Call Technical Service.
- Call Technical Service.