Leica Biosystems CM3050 S - Manual (Page 49)

49 Leica CM3050 S – Cryostat
6. Troubleshooting, applications tips
Problem Causes Remedies
Sections curl on anti-roll plate. - Anti-roll plate does not protrude
far enough beyond the knife
edge.
- Readjust correctly.
Scraping noise during section-
ing stroke and specimen return
stroke.
- Anti-roll plate protrudes too far
beyond the knife edge and is
scraping against the specimen.
- Readjust correctly.
Ondulated sections. - Knife damaged.
- Edge of anti-roll plate damaged.
- Use different part of the knife
or replace knife.
- Replace anti-roll plate.
Chatter marks form during
sectioning.
- Specimen insufficiently frozen
onto specimen disc.
- Specimen disc not clamped
tightly enough.
- Specimen holder ball joint not
clamped.
- Knife not clamped tightly enough.
- Selected section thickness too
thick - specimen has come off the
specimen disc.
- Specimen very hard and inhomo-
geneous.
- Blunt knife.
- Knife profile inappropriate for the
type of specimen to be sectioned.
- Wrong clearance angle.
- Refreeze specimen onto disc.
- Check disc clamping.
- Check ball joint clamping.
- Check knife clamping.
- Refreeze specimen onto disc.
- Increase section thickness;
if possible, reduce specimen
surface.
- Use different part of knife or
replace knife.
- Use knife with a different
profile.
- Readjust clearance angle
setting.
Condensation forms on anti-roll
plate and knife during cleaning.
- Brush, forceps, cloth or other
cleaning item too warm.
- Accessories and clean-
ing material to be used
pre-cooled only. - Store on
storage shelves inside the
cryochamber.