Leica Biosystems CM3050 S - Manual (Page 41)

41 Leica CM3050S – Cryostat
5.5.2 Freezing specimens onto specimen
discs with 90 ° prism (Instruments w/
specimen cooling only)
In instruments with specimen cooling, the 90°
prism, which is inserted into the specimen head,
is an additional option to freeze specimens at
maximum temperature .
Installing the 90° prism
• Loosen screw (3) at specimen head.
• Insert pin (1) of prism completely into recep-
tacle (2) in the specimen head.
Important: the entire rear surface of the
prism must fit tightly against the specimen
head surface!
• Retighten screw (3) at specimen head.
The prism now forms a horizontal surface for
quick specimen freezing.
Freezing specimens with the 90° prism
• Apply sufficient amount of embedding me-
dium onto ambient temperature specimen
disc, add specimen and orient.
• Insert specimen disc with specimen into the
receptacle (4) of the prism.
• Press to activate menu item ‘Fast Object
Freezing’ and activate function by pressig
(see chapter 5.3.4 ‘Menu functions’ item 4).
5. Operation
The specimen head is now automatically
cooled down to the lowest temperature (-50 °C).
• Once the specimen is frozen, remove the
disc from the prism and store it in the quick-
freeze shelf until inserting it into the speci-
men head.
• Press again to activate menu item ‘Fast
Object Freezing’ and deactivate the func-
tion by pressing once again (see chap-
ter 5.3.4 ‘Menu functions’ item 4).
The specimen head temperature now automati-
cally returns to the previously selected set val-
ue.
• Loosen screw (3) at specimen head and re-
move prism (1).
• Store prism inside the cryochamber.
5.5.3 Optional accessories for freezing
specimens onto specimen discs and
for removing frozen specimens
Mobile heat extractor
In addition to the stationary heat extractor (part
of standard delivery), a mobile heat extractor
is available as an optional accessory - see Ap-
pendix ‘Ordering information’ - accessories ):
• Store heat extractor in chamber to precool.
• Place onto specimen in quick-freeze shelf.
• Remove as soon as specimen is frozen.
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