Leica Biosystems CM3050 S - Manual (Page 50)

50 Instructions for Use V 1.7 RevC - 08/2014
6. Troubleshooting, applications tips
Problem Causes Remedies
Anti-roll plate damaged after ad-
justment.
- Anti-roll plate protrudes too far
beyond the knife edge. Adjust-
ment was done in the direction of
the knife.
- Replace anti-roll plate; in
future, lift away from the
knife when aligning.
- Handle anti-roll plate more
carefully.
Thick/thin sections. - Wrong temperature for the tissue
to be sectioned.
- Knife profile inappropriate for the
specimen to be sectioned.
- Ice build-up on the knife back.
- Handwheel rotation not uniform
or inappropriate rotation speed.
- Knife not clamped tightly enough.
- Specimen disc not clamped cor-
rectly.
- Embedding medium poured onto
cold specimen disc; specimen
comes off the disc once frozen.
- Blunt knife.
- Incorrect clearance angle.
- Specimen dried out.
- Select correct temperature.
- Wait until correct tempera-
ture has been reached.
- Use knife with different pro-
file (c or d) or possible switch
to disposable blade system.
- Remove ice.
- Adapt speed.
- Check knife clamping.
- Check disc clamping.
- Place embedding medium on
luke-warm specimen disc, in-
side the cryochamber place
the specimen onto the disc
and freeze in quick-freeze
shelf.
- Use different part of knife or
replace knife.
- Set correct clearance angle.
- Prepare new specimen.
Tissue sticks to the anti-roll plate. - Anti-roll plate too warm or incor-
rectly adjusted.
- Static electricity.
- Fat on corner or edge of anti-roll
plate.
- Knife rusty.
- Cool or readjust anti-roll
plate.
- Eliminate cause.
- Remove fat (alcohol).
- Remove rust.
Flattened sections curl up as soon
as anti-roll plate is lifted from the
knife.
- Static electricity or draft.
- Anti-roll plate too warm.
- Eliminate cause.
- Cool anti-roll plate.