7 setting the section thickness, Operation – Leica Biosystems CM3050 S User Manual

Page 39

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39

Leica CM3050S – Cryostat

5. Operation

Press this key to alternate between the

two functions.

illuminated:

Trimming mode is active

off:

Sectioning mode is active.

Selecting trimming thickness

• Activate trimming mode

• Select trimming thickness (= sectioning

thickness at which trimming is carried out)

by pressing

or

.

The selected thickness is displayed in the cor-

responding LED

:

Motorized trimming

• Select ‘Continuous stroke’ in functional area

A.

• Activate trimming by pressing RUN/STOP

and RUN/ENABLE (located in functional

area C) simultaneously or by depressing the

footswitch.

• To stop trimming press RUN/STOP or RUN/

ENABLE or depress the footswitch again.

Trimming thickness can be changed while trim-

ming is in motion by pressin

o

.

5.4.7 Setting the section thickness

• Activate sectioning mode

off).

• Press

or

to select section thickness.

Pressing one of these keys once makes the
display in control panel 1 switch to section
thickness indication (thickness value not yet
being altered). Pressing

or

further

starts to change the actual section thick-
ness setting.

Selectable section thickness settings:

0 -

2 µm in 0.5-µm steps,

2 - 10 µm in 1-µm steps,

10 - 20 µm in 2-µm

steps,

20 - 60 µm in 5-µm

steps,

60 - 100 µm in 10-µm

steps,

100 - 300 µm in 50-µm

steps.

5.4.6 Trimming and sectioning functions-

switching between trimming and

sectioning

Functional area B - all models

C T . . . . ° C O T . . . . ° C

µ 0 1 0 . 0

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