7 setting the section thickness, Operation – Leica Biosystems CM3050 S User Manual
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Leica CM3050S – Cryostat
5. Operation
Press this key to alternate between the
two functions.
illuminated:
Trimming mode is active
off:
Sectioning mode is active.
Selecting trimming thickness
• Activate trimming mode
• Select trimming thickness (= sectioning
thickness at which trimming is carried out)
by pressing
or
.
The selected thickness is displayed in the cor-
responding LED
:
Motorized trimming
• Select ‘Continuous stroke’ in functional area
A.
• Activate trimming by pressing RUN/STOP
and RUN/ENABLE (located in functional
area C) simultaneously or by depressing the
footswitch.
• To stop trimming press RUN/STOP or RUN/
ENABLE or depress the footswitch again.
Trimming thickness can be changed while trim-
ming is in motion by pressin
o
.
5.4.7 Setting the section thickness
• Activate sectioning mode
off).
• Press
or
to select section thickness.
Pressing one of these keys once makes the
display in control panel 1 switch to section
thickness indication (thickness value not yet
being altered). Pressing
or
further
starts to change the actual section thick-
ness setting.
Selectable section thickness settings:
0 -
2 µm in 0.5-µm steps,
2 - 10 µm in 1-µm steps,
10 - 20 µm in 2-µm
steps,
20 - 60 µm in 5-µm
steps,
60 - 100 µm in 10-µm
steps,
100 - 300 µm in 50-µm
steps.
5.4.6 Trimming and sectioning functions-
switching between trimming and
sectioning
Functional area B - all models
C T . . . . ° C O T . . . . ° C
µ 0 1 0 . 0