Leica Biosystems CM3050 S - Manual (Page 42)

42 Instructions for Use V 1.7 RevC - 08/2014
5. Operation
5.5.4 Inserting specimen discs into the
specimen head
• Lock handwheel in upper position.
If a knife has already been inserted
into the knife holder, cover knife edge
with knife guard!
• Loosen clamping screw (3).
• Insert specimen disc in opening (2) (rear
surface of specimen disc must fit tightly
against surface of specimen head).
• Tighten clamping screw (3).
5.5.5 Specimen orientation
• Open clamping lever (4) --> (4a).
• Orient specimen via orienting screws (1)
and (5).
• Retighten clamping lever (4).
Thermoblock
The thermoblock makes it easy to remove fro-
zen specimens from the specimen discs.
Do not store the thermoblock in-
side the cryochamber - store at room
temperature and only place into the
chamber for removing specimens from
discs.
• Place cap (9) on one of the two ends so that
the correct size opening (7) for the disc to
be inserted is exposed.
• Insert pin (6) of specimen disc (5) into the
opening (7).
• After about 20 seconds remove the fro-
zen specimen from the specimen disc (use
tweezers).
• If cap (9) fits too loosely, readjust with
screw (10). Do not tighten screw too much!
• Once the specimen has been detached from
the disc, remove the thermoblock from the
cryochamber.