Leica Biosystems CM3050 S - Manual (Page 53)

53 Leica CM3050S – Cryostat
7. Disinfection, cleaning and maintenance
7.1 Safety instructions on disinfection
and cleaning
When disinfecting the instrument,
make sure to observe the following
safety instructions:
• ‘Disinfection and Cleaning’
(Chapter 2.3.10)
• ‘Handling microtome knives/blades’
(Chapter 2.3.4)
• ‘Defrosting / handling frozen tissue’
(Chapter 2.3.7)
• ‘Frozen instrument parts and acces-
sories’ (Chapter 2.3.8)
• ‘Infectious / radioactive material’
(Chapter 2.3.9)
7.2 Spray disinfection with Leica Cryo-
fect
For practical spray disinfection, we recommend
Leica Cryofect which is fully effective at tem-
peratures in the negative range down to -20 °C.
• Set chamber temperature -20 °C or warmer.
• Remove specimens from the chamber and
store safely.
• Carefully remove knife / blade from knife
holder.
• Remove section waste tray from cryocham-
ber and empty.
• Remove remaining section waste from cryo-
chamber.
• Working accessories (brushes, specimen
holders etc.) can be disinfected inside the
cryochamber.
• Once the selected chamber temperature has
been reached, spray Leica Cryofect on the
surfaces and accessories to be disinfected.
• Allow to soak for 15 minutes.
• Use paper towels or another type of dispos-
able cleaning cloths to wipe up disinfectant
and remaining section waste.
• Dispose of paper towels together with sec-
tion waste.
• If previously altered, set chamber tempera-
ture back to its former value.
• Put specimens back into the cryochamber.