Leica Biosystems CM1100 - Manual (Page 23)

23 Leica CM1100 – Cryostat
8. Daily operation
8.5 Sectioning 8.6 Section transfer
Take care when removing the section - the
cutting edge is exposed!
Section transfer to a slide at room temperature
• Carefully approach a slide at room temperature to
the cut section.
The frozen section ‘flies‘ to the slide, rapidly thaws and
thus adheres to the slide surface making subsequent
orientation impossible.
Section transfer to a precooled slide
• Carefully apply the section to the slide by gentle
brushing with a soft brush.
• Orient and flatten the section on the slide surface
with a brush.
• To ensure the section tightly adheres to the slide
surface, warm the cold slide by placing a finger
against the underside of the slide directly under-
neath the section.
Fig. 23
1
• Select a section thickness with the section thick-
ness control knob (1) at the top of the microtome.
• Decrease the section thickness continually to the
required value.
After changing from one section thickness to
another, the first two or three sections should
be rejected!
• Make sure the section smoothly slides down be-
tween the anti-roll plate and blade.
• Remove the anti-roll guide from the knife for remov-
ing and applying a section to a microscope slide.
The section can be transferred either to a precooled
microscope slide or to a microscope slide at room tem-
perature.