Leica Biosystems CM1100 - Manual (Page 26)

26 Instruction manual, V1.2 – 06/2006
9. Trouble shooting
- Refreeze specimen onto the disc.
- Check disc clamping.
- Check clamping.
- Refreeze specimen onto the disc.
- Increase section thickness; reduce
specimen surface area if neces-
sary.
- Use different part of cutting edge,
or replace blade.
- Store all tools in the cryochamber.
- Raise plate when aligning.
Be more careful next time.
- Select correct temperature.
Wait until the correct temperature
has been reached.
- Remove ice.
- Adapt speed.
- Check clamping.
- Check clamping.
- Apply cryocompound on warm
disc; mount specimen and freeze.
- Use different part of cutting edge,
or replace blade.
- Dry microtome thoroughly.
- Cool down anti-roll plate or
reposition plate.
- Remove static electricity.
- Remove fat with alcohol/acetone.
- Remove rust.
- Specimen insufficiently frozen onto
the specimen disc.
- Specimen disc not clamped tightly.
- Blade not clamped tightly enough.
- Specimen has been sectioned too
thickly and has detached from the
disc.
- Very hard, inhomogeneous speci-
men.
- Blunt cutting edge.
- Brush, forceps and/or cloth too
warm.
- Plate too high above the cutting
edge. Adjustment was carried out
in direction of the cutting edge.
- Temperature incorrect for the
tissue to be cut.
- Ice buildup on blade back.
- Handwheel speed not uni-
form.
- Blade not clamped tightly enough.
- Specimen disc not clamped tightly.
- Cryocompound applied to cold
specimen disc; specimen detached
from the disc after freezing.
- Blunt cutting edge.
- Microtome not properly dried
before reinstallation.
- Anti-roll plate is too warm or
incorrectly positioned.
- Static electricity.
- Fat on the corner or edge of the
anti-roll plate.
- Rusty blade.
Chatter during sectioning
Condensation on anti-roll plate and
blade during cleaning
Anti-roll plate damaged after
adjustment
Thick-thin sections
Tissue sticks or crumbles on the anti-
roll plate
Problem Possible causes Remedies