Leica Biosystems CM1100 - Manual (Page 27)

Leica CM1100 – Cryostat 27
9. Trouble shooting
Flattened sections curl up when anti-
roll plate is picked up
Sections tear
Inconsistent or insufficient specimen
feed
Specimen disc cannot be removed
Cryostat inoperational; no display
indication
No or insufficient refrigeration
- Static electricity or air currents.
- Anti-roll plate too warm.
- Temperature too low for the tissue
to be cut.
- Blunt part, dirt, dust, frost or rust
on the blade.
- Leading edge of anti-roll plate
damaged.
- Hard particles in the tissue.
- Blade back dirty.
- Microtome was not entirely dry
when switching on refrigeration;
consequently ice built up in the
micrometer feed system.
- Defective microtome.
- Moisture on the underside caused
the disc to freeze to the quick-
freeze shelf or specimen head.
- Mains plug not properly con-
nected.
- Overcurrent protection of the
mains switch has responded.
- Fuse defective.
- Inappropriate installation site.
- Required minimum distance of 10
cm on all sides not observed.
- Compressor ventilation grids dirty
or covered.
- Instrument has regularly been
switched off overnight; therefore
an automatic defrost cycle did not
take place.
- Compressor defective.
- Remove static electricity.
- Cool down the anti-roll plate.
- Increase temperature and wait.
- Remove cause.
- Replace the plate.
- Cannot be corrected.
- Clean.
- Remove the microtome and dry
thoroughly before reinstallation.
- Call technical service.
- Apply concentrated alcohol to the
contact point.
- Connect the mains plug is properly.
- Switch on again with the mains
switch after approx. 5 minutes.
- Replace 12 V fuse on rear of
instrument.
- Comply with installation site
requirements.
- Comply with installation site
requirements.
- Remove obstruction or remove
dust with brush or vacuum cleaner.
- Start a manual defrost cycle.
- Call technical service.
Problem Possible causes Remedies