Cleaning and disinfection, 1 cleaning, 2 spray disinfection with leica cryofect – Leica Biosystems CM1100 User Manual

Page 28: Cleaning, Spray disinfection with leica cryofect

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Instructions for use V1.3 RevB – 09/2012

10.

Cleaning and disinfection

Remove frozen section waste from the cold cry-
ostat.

Remove the waste trays and empty.

Do not use organic solvents or any other ag-
gressive substances for cleaning and disin-
fection!
We strongly recommend the use of Leica
Cryofect disinfectant spray!
Only use alcohol or common disinfectants
based on alcohol!

10.1 Cleaning

10.2 Spray disinfection with Leica Cryofect

Keep only in original container and up to
25 °C!

Wear appropriate protective clothing when
cleaning and disinfecting!
Disinfect the instrument and accessories ev-
ery day!

Effectiveness of the disinfectant down to -20
°C experimentally tested.

Highly flammable (VbF: B)!
Flash-point: 18 °C (DIN 51755)
Keep away from sources of ignition!
Do not spray into flames!
Attention with alcohol-sensitive surfaces!

1. Select a cryochamber temperature value down to -

20 °C.

2. Remove the knife or blade from the knife holder.
3. Remove all samples, microscope slides and tools

from the cryochamber.

4. Remove debris from the cryochamber.

Allow the cryochamber to reach the previously se-
lected temperature.

Once the selected temperature is reached, either

5a. spray the disinfectant evenly on the contaminated

surfaces- the surfaces should be covered with an
even layer - or

5b. soak a cloth with disinfectant and apply it on the

contaminated surfaces.

6. Allow a reaction time of no less than 15 minutes.
7. Wipe it off with a tissue.
8. Dispose of tissue in compliance with the ruling

waste disposal regulations of your institution.

9. Set the cryochamber temperature to the originally

selected value.

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