Leica Biosystems RM2235 - Manual (Page 67)

65 Leica RM2235
7. Troubleshooting
Problem Possible cause Corrective action
• When using cassettes, particu-
larly thin-walled cassettes, from
a company other than Leica or
Surgipath, the cassette may be-
come deformed or other clamp-
ing problems may arise. If while
trying to clamp the cassette you
realize that it is not securely
clamped into place, an alterna-
tive tensioning clamp must be
used.
• The blade is dull.
• The pressure plate is damaged
or incorrectly adjusted.
• Clearance angle of the knife/
blade too small.
• Laterally displace the knife hold-
er or insert a new blade.
• Insert a new pressure plate or
use a new knife holder.
• Readjust the pressure plate.
• Systematically try several clear-
ance angle adjustments, until the
optimum angle is found.
2. Section compression
The sections are very com-
pressed, show folds or are
squeezed together.
• The blade is dull.
• The specimen is too warm.
• The sectioning speed is too fast.
• Use another area of the blade or
a new blade.
• Cool the specimen before sec-
tioning.
• Reduce the sectioning speed.
3. "Stripes" in sections
For knife holder E
• There is an accumulation of par-
affin on the rear pressure plate
of the knife holder.
• Remove paraffin from this area
on a regular basis.