Bio-Rad Experion Protein Analysis Kits User Manual
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1.2.5 Prime the Chip
Before priming the chip, ensure that all samples, the RNA ladder, and other reagents are
prepared and ready to be loaded. If a chip is not used within 5 min of priming and loading,
reagents may evaporate, leading to poor results. Use the narrow-bore pipet tips that are
supplied with the kit (or equivalent tips) when loading samples or reagents into the chip.
1.
Pipet 9 µl GS into the well labeled GS (gel priming well) (Figure 1.1). Insert the pipet tip
vertically and to the bottom of the well when dispensing. Dispense slowly, and do not
expel air at the end of the pipetting step.
Note: Placing the pipet tip at the edge of the well or allowing the gel to slide down the wall
of the well may lead to bubble formation at the bottom of the well. Remove any bubbles at
the bottom of the well by removing the GS and loading a new aliquot. For help with chip
loading, refer to the Experion Training Video: Chip Loading.
Fig. 1.1. Experion RNA StdSens chip. The locations of the gel priming well (GS) and alphanumeric
priming code are indicated.
2.
Set the pressure setting to B and the time setting to 1, as specified by the alphanumeric
code on the chip (Figure 1.1).
3.
Open the Experion priming station by pressing down on the lever on the front.
4.
Place the chip on the chip platform, matching the arrow on the chip with the alignment
arrow on the chip platform. A post on the chip prevents insertion in the wrong position.
Do not force the chip into position.
5.
Carefully close the priming station by pressing down on the lid. The lid should snap
closed.
6.
Press the Start button. A “Priming” message appears on the screen of the priming
station, the priming station pressurizes, and the timer begins to count down. Priming
requires approximately 30 sec to complete. Do not open the priming station during the
countdown.
7.
An audible signal and “Ready” message indicate that priming is complete. Open the
priming station by pressing down on the release lever.
Note: If the lid sticks, press down on it while pressing down on the release lever.
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