Bio-Rad Experion Protein Analysis Kits User Manual
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Fig. 1.3. Bubble formation during loading of Experion Pro260 chips. Left, example of bubbles
trapped at the bottom of wells. The GS and G wells and sample wells 1, 3, and 4–6 contain no solution.
Well 10 is filled properly and has no bubbles; large bubbles have formed at the bottom of wells 8 and 9
and in the ladder well (L). Note the difference in the diameter of the light-colored circles in wells 10 and
L. Right, example of bubble formation at the surface of wells. Small bubbles have formed at the surface
of the three GS wells on the right side of the chip, and the rest of the wells have no bubbles. Surface
bubbles should not cause problems during a run, but bubbles at the bottoms of wells must be removed.
7.
Place the chip in the Experion vortex station. Turn on the vortexer, which operates for
60 sec and then automatically shuts off. Remove the chip when the vortexer stops.
8.
Place the loaded chip into the Experion electrophoresis station and start the run. Run
a loaded chip immediately (within 5 min) or excess evaporation may occur, leading to
poor results or a chip performance error.
1.2.7 Run the RNA StdSens Analysis
1.
Open the lid of the electrophoresis station by pulling the release latch. Place the
primed and loaded chip on the chip platform and close the lid.
2.
In the Experion software toolbar, click the New Run button . The
New Run
screen opens (Figure 1.4).
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