Bio-Rad Experion Protein Analysis Kits User Manual
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1.3 Data Analysis
Evaluate the performance of Test 1 and the analysis of the data by Experion software.
1.
Evaluate the virtual gel to ensure that all lanes (samples) are visible. Check that the
lower marker is present in each sample (indicated by pink triangles) and that it is
aligned across all lanes (Figure 1.6).
2.
Modify the gel view by changing the fluorescence intensity scale. By default, Experion
software displays the virtual gel and electropherograms as “scaled to local”, which
means the gel lane or trace of each sample is scaled to the highest peak intensity
detected in that sample. This is the best method for viewing an electropherogram.
To adjust the fluorescence intensity scale so that the virtual gel most closely resembles
a gel-based separation, select Graph > Scale to Global. The virtual gel appears as it
does in Figure 1.6, right panel. All lanes are scaled to the highest peak found across
the entire chip, which enables comparison of the concentration differences among the
samples in the virtual gel
Fig. 1.6. Virtual gel generated by Test 1. Left, virtual gel “scaled to local”. Right, virtual gel “scaled to
global”. L, RNA ladder; lanes 1–12, 100 ng/µl total RNA sample.
13
28S
18S
Lower marker
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