Bio-Rad Experion Protein Analysis Kits - Manual (Page 19)

4. Examine the separation of the total RNA sample. Click on the sample name in the
project tree or on a lane in the virtual gel. The electropherogram appears and should
have the following features (Figure 1.8):
• A lower marker peak at ~21 sec, at least 5 fluorescence units above the baseline,
and distinguishable from the sample peaks
• Flat baseline
• Well-resolved 18S and 28S rRNA peaks
Fig. 1.8. Separation of the 100 ng/µl mouse liver total RNA sample (scaled to local). Results shown
are typical for separations of total RNA from eukaryotic sources. The two most prominent peaks, aside
from the lower alignment marker, represent 18S and 28S ribosomal RNA (rRNA).
5. Evaluate the data analysis performed by Experion software. Click on the Results tab
and click on lane 2 in the virtual gel. The Results table for sample 2 opens (Figure 1.9).
View the RNA concentration given in the Results table. In the example in Figure 1.9,
the total concentration of the sample is 112.14 ng/µl.
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