Bio-Rad Experion Protein Analysis Kits - Manual (Page 6)

The Experion RNA StdSens starter kit was designed to introduce new users to RNA analysis
with the Experion system as well as to enable troubleshooting, performance maintenance,
or performance qualification of the Experion system. The starter kit is derived from the
Experion RNA StdSens analysis kit, which is used with the Experion system to determine
total RNA and mRNA integrity, purity, and concentration at nanogram levels. A second
RNA analysis kit (the Experion RNA HighSens analysis kit) is used for analysis at
picogram levels. All kits include the Experion RNA ladder, which has been optimized for
automated electrophoresis on the Experion system. They also feature the Experion RNA
loading buffer, which contains a 50 bp marker that is used for the proper alignment of
samples to the RNA ladder. The starter kit contains the same components as the Experion
RNA StdSens analysis kit in addition to other supplies that allow a new user to start using
the system immediately.
RNA analysis with the Experion system compares favorably with other, more conventional
methods for RNA quantitation, such as UV spectroscopy, with regards to assay linearity,
reproducibility, and accuracy. However, in contrast with UV spectroscopy, Experion analysis
is relatively unaffected by reagents common to RNA preparation. The additional microfluidic
separation step also affords the unique ability to visually evaluate the RNA sample for
degradation and for the presence of copurifying nucleic acids, such as genomic DNA and
tRNA (Woo and Strong 2006)
1 .
For more information on RNA analysis using the Experion system, refer to Appendix A of
this manual.
Detection and Quantitation Ranges of the Experion RNA Analysis Kits
RNA StdSens Analysis Kit RNA HighSens Analysis Kit
Total RNA mRNA Total RNA mRNA
Detection range 5–500 ng/µl 25–250 ng/µl 100–5,000 pg/µl 500–5,000 pg/µl
Quantitation range 25–500 ng/µl 25–250 ng/µl 200–5,000 pg/µl 500–1,000 pg/µl
1 Woo L and Strong W, RNA quantitation: A comparative analysis using the Experion automated electrophoresis system,
RiboGreen reagent, and ultraviolet spectroscopy, Bio-Rad bulletin 5420 (2006)
2
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