Bio-Rad Experion Protein Analysis Kits - Manual (Page 13)

8. Remove the chip from the priming station, turn it over, and inspect the microchannels
for bubbles or evidence of incomplete priming. If the chip is primed properly, the
microchannels are difficult to see (it may be helpful to compare a primed chip to a
new, unused chip). If you detect a problem, such as a bubble or incomplete priming,
prime a new chip.
9. Place the chip on a clean surface for loading.
1.2.6 Load the Chip
1. Pipet 9 µl GS into the other well labeled GS (NOT the gel priming well) (Figures 1.1
and 1.2).
2. Pipet 9 µl filtered gel into the well labeled G (Figures 1.1 and 1.2).
3. Pipet 5 µl loading buffer (yellow cap) into each sample well (1–12) and into the ladder
well (L).
Note: Use a new pipet tip for each delivery to prevent contamination of the loading
buffer stock. Alternatively, remove 70 µl of loading buffer into an RNase-free tube and
pipet 5 µl to each well from this volume.
4. Pipet 1 µl denatured RNA ladder into the well labeled L. Every chip must have the
RNA ladder loaded into the ladder well labeled L.
5. Pipet 1 µl sample into each of the sample wells (1–12). Every sample well (1–12)
must contain solution.
Fig. 1.2. Chip layout for Experion RNA StdSens starter kit Test 1. S1, sample; GS, gel-stain solution;
G, RNA gel; L, RNA ladder.
6. Inspect all wells to make sure that there is no excessive bubble formation from pipetting.
Hold the chip above a light background and look down through the wells (Figure 1.3).
Dislodge any bubbles at the bottom of the well with a clean pipet tip or by removing
and reloading the solution.
S1 S1 S1 G
S1 S1 S1 GS
S1 S1 S1 GS
S1 S1 S1 L
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