Bio-Rad Experion Protein Analysis Kits - Manual (Page 28)

How Experion Software Analyzes RNA
Data Presentation
As the photodiode detects fluorescent signals from the dye-RNA complexes, Experion
software converts the signal into an electropherogram (a plot of fluorescence vs. time).
Experion RNA electropherograms differ depending on the type of sample analyzed
(eukaryotic or prokaryotic total RNA or mRNA), but they generally have the following features:
Sample peak(s) –– signal(s) generated by the sample RNA fragment(s)
Lower marker (LM) –– signal generated by the internal lower (50 bp) marker, which is
included in the loading buffer and used to align the separations across all wells in the chip
Electropherogram generated by Experion RNA StdSens analysis of eukaryotic total RNA. Results
shown are typical for separations of total RNA from eukaryotic sources. The two most prominent peaks,
aside from the lower alignment marker, represent 18S and 28S ribosomal RNA (rRNA).
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