Bio-Rad Experion Protein Analysis Kits User Manual
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11. Plot the results as a function of sample concentration (Figure 2.7). The overall trend
should be linear.
Fig. 2.7. Plot of concentration calculated by Experion software as a function of expected
concentration.
Expected Performance of Test 2. Based on results obtained using a total of 6 chips.
Measurement
Typical Results
Quantitation reproducibility
%CV £ 20% for total RNA (25–500 ng/µl)
If your results do not appear as they do in these examples, refer to the Troubleshooting
section of the Experion RNA StdSens and Experion system instruction manuals or contact
Bio-Rad technical support. Typically, problems with reproducibility are due to problems with
pipetting or chip loading. For tips on optimizing your results, refer to the Experion Training
Video: Chip Loading and the Essential Practices and Troubleshooting sections of the
Experion system instruction manual.
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