Bio-Rad Bio-Plex Pro™ Magnetic Cell Signaling Assays - Manual (Page 14)

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2. Plan the Plate Layout
Prior to running the assay, determine the total number of wells in the
experiment using the Plate Layout Template on page 30 or the Plate
Formatting tab in Bio-Plex Manager. A suggested plate layout is shown in
Figure 2, with all conditions in duplicate. Please note that the Bio-Plex 200
instrument reads the plate vertically.
Table 3. Selection guide for Bio-Rad cell lysate controls (continued).
Total Target Lysate Control Catalog #
Total Akt Total mTOR
Total Erk 1/2 Total p38 MAPK
Total GSK-3b Total p70 S6 Kinase
Total IkB-a Total PTEN
Untreated HeLa 171-YZT002
Total JNK Total Smad2
Total MEK1 Total IGF-1R
Total Btk H
2
O
2
-treated Ramos 171-YZ0011
Total c-Jun
Total CREB
Untreated HEK-293 171-YZT001
Total HER-2 EGF-treated SK-BR-3 171-YZ0003
Total Src Src-transfected NIH3T3 171-YZ0013
Total ZAP-70 H
2
O
2
-treated Jurkat 171-YZ0012
Negative control for all total target assays Detection antibody diluent
House Keeping Protein
Human GAPDH b-Actin Untreated HeLa 171-YZT002
Negative control for all total target assays Detection antibody diluent