Bio-Rad Bio-Plex Pro™ Magnetic Cell Signaling Assays - Manual (Page 6)

4
Quantity***
1 tube
1 tube
1 bottle (50 ml)
1 bottle (50 ml)
1 vial
1 bottle (330 ml)
1 bottle (10 ml)
1 bottle (40 ml)
1 tube
1 plate
1 pack of 4
1 booklet
50 μg per vial
50 μg per vial
Kit Components and Storage
Table 1. Components required for a complete 1 x 96-well cell signaling assay.
Note that custom x-Plex
™ assays include a premixed (multiplex) set of beads and
detection antibodies in an all-in-one kit.
* Users can mix compatible singleplex sets to create their own multiplex assays.
** Refer to Table 3 to identify the appropriate controls for your phosphoprotein or total target
of interest.
*** Volumes shown are approximate.
Storage and Stability
Kit contents should be stored at 4°C and never frozen. Coupled magnetic
beads and streptavidin-PE should be stored in the dark. All components
are guaranteed for a minimum of six months from the date of purchase
when stored as specified.
Component
Singleplex* or Multiplex Set
Coupled magnetic beads (20x)
Detection antibodies (20x)
Cell Signaling Reagent Kit (catalog #171-304006M)
Cell wash buffer
Cell lysis buffer
Cell lysis factor QG
Wash buffer
Detection antibody diluent
Resuspension buffer
Streptavidin-PE (100x)
Flat bottom plate
Sealing tape
Assay Quick Guide
Bio-Rad Cell Lysate Controls (optional)**
Positive control (treated or untreated)
Negative control (phosphatase treated)