Bio-Rad Bio-Plex Pro™ Magnetic Cell Signaling Assays - Manual (Page 7)

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Item
Bio-Plex Pro ™ Assays Quick Guide 3
Bio-Plex ® 200 system or Luminex system with HTF
Bio-Plex validation kit
Run monthly
Bio-Plex calibration kit
Run daily to standardize fluorescence signal
Bio-Plex Pro wash station
For use with magnetic bead-based assays only
Bio-Plex handheld magnetic washer
For magnetic bead–based assays only
Bio-Plex Pro flat bottom plates (forty 96-well plates)
For magnetic separation on the Bio-Plex Pro wash station
Microtiter plate shaker
IKA MTS 2/4 shaker for 2 or 4 microplates
or
Barnstead/Lab-Line Model 4625 plate
shaker (or equivalent capable of 300–1,100 rpm)
Aurum ™ vacuum manifold
For vacuum filtration
BR-2000 vortexer
Reagent reservoirs, 25 ml
For capture beads and detection antibodies
Reagent reservoir, 50 ml (for reagents and buffers)
Pall Life Science Acrodisc: 32 mm PF syringe filter
(0.45 µm Supor membrane)
DC ™ protein assay kit II
Phenylmethylsulfonyl fluoride (PMSF)
Dimethyl sulfoxide (DMSO)
Kontes tissue grinder
Other: Polypropylene tubes for reagent dilutions, calibrated pipets, pipet tips, sterile distilled
water, aluminum foil, paper towels, 1.5 or 2 ml microcentrifuge tubes, and a standard flat
bottom microplate (for calibrating vacuum manifold).
Ordering Information
Bulletin #10024930 (download
at www.bio-rad.com/bio-plex)
Bio-Rad catalog #171-000205
Bio-Rad catalog #171-203001
Bio-Rad catalog #171-203060
Bio-Rad catalog #300-34376
Bio-Rad catalog #171-020100
Bio-Rad catalog #171-025001
IKA catalog #320-8000
VWR catalog #57019-600
Bio-Rad catalog #732-6470
Bio-Rad catalog #166-0610
VistaLab catalog #3054-1002
or
VistaLab catalog #3054-1004
VistaLab catalog #3054-1006
Pall Life Sciences
catalog #4654
Bio-Rad catalog #500-0112
Sigma catalog #P7626
Sigma catalog #D2650
VWR catalog #KT885000-0002
Table 2. Recommended materials.