Bio-Rad Bio-Plex Pro™ Magnetic Cell Signaling Assays User Manual
Page 26
24
9. Click Enter Controls Info, and for user-specified controls, select
an analyte from the dropdown menu, then enter a description and
concentration. Repeat for each additional analyte in the assay.
For Bio-Rad cell lysate controls, format the appropriate wells as controls,
enter descriptions, but leave the concentrations blank. Alternatively, both
blanks and controls can be formatted as samples with clear descriptions.
Phosphoprotein Targets Bead Region
Akt (Ser
473
) 75
Akt (Thr
308
) 75
ATF-2 (Thr
71
) 20
BAD (Ser
136
) 26
Btk (Tyr
223
) 39
c-AbI (Tyr
245
) 45
c-Jun (Ser
63
) 56
CREB (Ser
133
) 19
EGFR (Tyr
1068
)
44
EGFR (Tyr
1173
)
44
Erk1/2 (Thr
202
/Tyr
204
, Thr
185
/Tyr
187
) 38
GSK-3a/b (Ser
21
/Ser
9
) 18
HER-2 (Tyr
1248
) 30
HSP27 (Ser
78
) 51
IGF-IR (Tyr
1131
) 43
IR-b (Tyr
1146
) 43
IRS-1 (Ser
636
/Ser
639
) 76
IkB-a (Ser
32
/Ser
36
) 67
JNK (Thr
183
/Tyr
185
) 34
Lyn (Tyr
507
) 33
MEK1 (Ser
217
/Ser
221
) 27
Phosphoprotein Targets Bead Region
mTOR (Ser
2448
) 46
NF-kB p65 (Ser
536
)
37
p38 MAPK (Thr
180
/Tyr
182
) 36
p53 (Ser
15
) 53
p70 S6 Kinase (Thr
389
) 55
p70 S6 Kinase (Thr
421
/Ser
424
) 55
p90 RSK (Ser
380
) 35
PDGFR-a (Tyr
754
) 28
PDGFR-b (Tyr
751
) 57
Pl3K p85 (Tyr
458
) 54
PTEN (Ser
380
) 22
S6 ribosomal protein (Ser
235
/Ser
236
) 74
Smad2 (Ser
465
/Ser
467
) 14
Src (Tyr
416
) 42
Stat1 (Tyr
701
) 61
Stat3 (Ser
727
) 52
Stat3 (Tyr
705
) 52
Syk (Tyr
352
) 65
VEGFR-2 (Tyr
1175
) 29
ZAP-70 (Tyr
319
) 64
Total Targets
Bead Region
Akt
75
Btk 39
c-Jun 56
CREB 19
Erk 1/2
38
GSK3b
18
HER-2
30
IGF-1R 43
IkBa
67
Total Targets
Bead Region
JNK
34
MEK1
27
mTOR 46
p38 MAPK
36
p70 S6 Kinase
55
PTEN 22
Smad2
14
Src
42
ZAP-70 64
Housekeeping Proteins
Bead Region
Human GAPDH
21
Housekeeping Proteins
Bead Region
b-Actin 47
Table 12. Bead regions for cell signaling assays.