Bio-Rad Bio-Plex Pro™ Magnetic Cell Signaling Assays - Manual (Page 16)

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3. Prepare the Wash Method
The cell signaling assays are compatible with both magnetic separation
and vacuum filtration methods. However, for best results, we recommend
performing the assays in a flat bottom plate with magnetic separation.
Setting up the Bio-Plex Handheld Magnetic Washer
Place an empty flat bottom plate on the magnetic washer by sliding it
under the retaining clips. Push the clips inward to secure the plate. Make
sure the plate is held securely. For detailed instructions, refer to the user
guide (bulletin 10023087).
Setting up the Bio-Plex Pro
The wash station should be primed before use. See bulletin 5826.
1. Install the appropriate plate carrier on the wash station.
2. Use the Prime procedure to prime channel 1 with wash buffer.
Note:
n
Before using the Bio-Plex Pro wash station, make sure to define/edit a
program with the correct settings for cell signaling assays (Table 5)
n
Existing cytokine assay programs: MAGx2, MAGx3, VACx2, and VACx3
should not be used
Refer to the wash station instruction manual (bulletin 10013125), or
contact Bio-Rad Technical Support for more information on defining,
editing, or importing wash station programs.
Table 4. Summary of compatible wash stations and plate types.
Wash Method
Wash Station Assay Plate
Magnetic separation Bio-Plex Pro Flat bottom plate
Bio-Plex handheld magnetic washer
Vacuum filtration Vacuum manifold (manual) Filter plate