Bio-Rad Bio-Plex Pro™ Magnetic Cell Signaling Assays - Manual (Page 25)

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protocol. To create a new protocol, select File, then New from the main
menu. Locate and follow the steps under Protocol Settings.
6. Click Describe Protocol and enter information about the assay
(optional).
7. Click Select Analytes and create a new panel. Visually confirm the
selected analytes and proceed to step 8.
a. Click the Add Panel button in the Select Analytes toolbar.
Enter a new panel name. Select Bio-Plex Pro Assay (Magnetic)
or MagPlex Beads (Magnetic) from the assay dropdown list.
If using Bio-Plex Manager version 5.0 or lower, select MagPlex
from the assay dropdown list.
b. Click the Add button. Enter the bead region number and name for
the first analyte. Click Add Continue to repeat for each analyte in
the assay. Refer to the bead regions listed in Table 12.
c. Click the Add button when the last analyte has been added and
click OK to save the new panel.
d. Highlight analytes from the Available list (left) and move to the
Selected list (right) using the Add button. To move all analytes at
once, simply click the Add All button.
e. If some of the analytes need to be removed from the Selected
list, highlight them and select Remove. If desired, it is possible to
rename the panel by clicking Rename Panel and entering a new
panel name.
8. Click Format Plate, and format the plate according to the plate
layout created in Section 2, Plan the Plate Layout. To modify the plate
layout, follow the steps below (see Figure 3).
a. Select the Plate Formatting tab.
b. Select the blank icon B and drag the cursor over all the wells
that contain blanks. Repeat this process for Controls
C
and
Samples
X
. Note that Bio-Plex Manager automatically
subtracts the blank MFl value from all other assay wells.