Bio-Rad Bio-Plex Pro™ Magnetic Cell Signaling Assays - Manual (Page 30)

28
Troubleshooting Guide
This troubleshooting guide addresses problems that may be encountered
with Bio-Plex Pro
™ assays. If you experience any of the problems listed
below, review the possible causes and solutions provided. Suboptimal
assay performance may also be due to the Bio-Plex
® suspension array
reader. To eliminate this possibility, use the Bio-Plex validation kit to assist
in determining if the array reader is functioning properly.
Table 13. Troubleshooting guide.
Problem Possible Causes Possible Solutions
Low signals for Sample protein concentration Verify sample protein concentration
experimental too low or too high is within assay range. Optimization
samples of protein concentration may be
needed based on targeted protein
expression level
Poor cell lysate quality Prepare fresh lysate accordingly
Low signals for Incorrect dilution of detection Check the calculations and be
experimental samples antibody or streptavidin-PE careful to add the correct volumes
and Bio-Rad cell
lysate controls
Expired Bio-Plex reagents Check that reagents have not
were used expired. Use new or non-expired
components
Incorrect incubation Incubations should be at room
temperature temperature (20–22°C)
Insufficient incubation time Adhere to the recommended
incubation time
Beads lost Use recommended magnetic
washer settings with correct
magnet soaking time
continues