Bio-Rad Bio-Plex Pro™ Magnetic Cell Signaling Assays - Manual (Page 15)

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1. Assign blank, negative control, positive control, and samples
accordingly.
Note: When designating blank using B , Bio-Plex Manager
software will automatically subtract the blank MFI value from all other
assay wells.
2. Once the total number of wells is known, see Tables 6–9 or the
Calculation Worksheet on pages 32–33 to determine the required
volumes of beads, detection antibodies, and streptavidin-PE to use.
Note that 20–25% excess volume is included in the calculations to
compensate for transfer loss.
Legend
B Blank
X Samples
C Controls
Fig. 2. Suggested plate layout. For detailed instructions on
plate formatting in Bio-Plex Manager, see Section 5, Read the Plate.