Table of Contents
Introduction 1
Principle 2
Kit Components and Storage
4
Recommended Materials
5
Assay Workflow
6
lmportant Considerations
7
Detailed Instructions
8
1
1. Prepare the Samples 8
2
2. Plan the Plate Layout 12
3
3. Prepare the Wash Method 14
4. Run the Assay 16
5. Read the Plate 21
Troubleshooting Guide
28
Plate Layout Template
31
Calculation Worksheet
32
Safety Considerations
34
Legal Notices
Ordering Information
35