Bio-Rad Bio-Plex Pro™ Magnetic Cell Signaling Assays - Manual (Page 8)

6
Assay Workflow
Prewet wells (for filter plate only)
Add 50 μl 1x beads to wells
Wash 2x
Add 50 μl samples, controls, and blanks;
incubate overnight at RT with shaking
Wash 3x
Add 25 μl 1x detection antibody,
incubate 30 min at RT with shaking
Wash 3x
Add 50 μl 1x streptavidin-PE,
incubate 10 min at RT with shaking
Wash 3x
Add 125 μl of resuspension buffer,
shake for 30 sec
Acquire data