Bio-Rad BioLogic LP System - Manual (Page 14)

2. Connect the cartridge between the injector valve outlet and the UV
monitor flow cell, as shown in the system plumbing diagram (Fig. 5).
3. Install 50 cm of 1.6 mm ID tubing for a 1.0 ml sample loop. The male
luer fittings on each end of the tubing are used to connect to the injector
valve.
Section 13
Purging the System
1. Place the inlet tubes for “A” and “B” buffers in their respective buffer
containers.
2. Turn the system power on—you will see the “manual” screen. Note:
that the conductivity and UV absorbance values are displayed on the
manual screen.
3. Press the PUMP instrument soft key.
4. Press PURGE soft key.
Note: if a column bypass valve is connected to your system switch it to
bypass, otherwise, remove the column from the flow path during the
purge.
5. Press the BUFFER soft key, use the Previous/Next keys to select
buffer “B”, press OK—purge the system with “B” buffer until air is
expelled, and the conductivity reading stabilizes. Note the conductivity
of “B” buffer here: ______ mS. (You will use this information later.)
6. Press the BUFFER soft key, use the Previous/Next keys to select
buffer “A”, press OK—purge the system with “A” buffer until air is
expelled, and the conductivity reading stabilizes. Note the conductivity
of “A” buffer here: ______ mS. (You will use this information later.)
7. Press the STOP soft key.
8. If the column was removed, reconnect the column to the system.
Section 14
Conductivity Monitor Calibration and
Setting Minimum and Maximum Range
1. Calibrate the conductivity flow cell by:
a. Press the COND soft key on the BioLogic LP instrument front
panel.
10
4006073D.qxp 8/17/2009 7:45 AM Page 10