Bio-Rad BioLogic LP System - Manual (Page 18)

Section 19
Setting the Alarm
The alarm sounds after 3 minutes to remind you to turn the valve back
to the load position. (Proper technique is to switch the sample loop out of
the flow path during gradient elution; the starter kit separation will be
successful either way.)
1. Press the ALARM soft key.
2. Press ADD.
3. Enter a time of 3 minutes for Alarm 1 and check that Hold Methods is
set to NO.
4. Press OK twice.
Section 20
Entering the Fraction Collector Program
1. Press the FRAC COLL soft key on the front panel of the BioLogic LP
workstation.
2. Select ALL.
3. Enter a fraction size of 2 minutes (2 ml if your method is based on
volume).
4. Press OK twice, then select DONE..
5. Press SAVE, name method “DEMO 1”, press DONE.
Section 21
Loading the Sample Loop
1. Turn the MV-6 injector valve knob counterclockwise as far as it will go
as shown in Figure 7.
2. Draw 1.5 ml of the protein standard mixture into the syringe making
sure to push out as much air from the syringe before inserting in to the
injector valve.
3. Insert the syringe in the top port of the MV-6 injector valve and fill the
sample loop.
4. Leave the syringe in the port after you have injected the sample
as this prevents the sample from siphoning out of the loop to waste.
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