Bio-Rad BioLogic LP System - Manual (Page 22)

3. Enter a pump program as described above—you may wish to recall
your earlier method and edit.
Step 1. Buffer “A”, step Length 1 minute, flow rate 1.5 ml/minute
Step 2. Buffer “C”, step length 3.5 minutes, flow rate 1.5 ml/minute
Step 3. Buffer “A”, step length 1 minute, flow rate 1.5 ml/minute
Step 4. Gradient 0% to 50% “B”, step length 10 minutes, flow rate
1.5 ml/minute
Step 5. Buffer “B”, step length 6 minutes, flow rate 1.5 ml/minute
Step 6. Buffer “A”, step length 6 minutes, flow rate 1.5 ml/minute
4. For fraction collection, use “Collect All” with a fraction size of 2 minutes.
14 tubes will be required.
5. Perform final checks and run method as described above.
Note: The first peak (“void” peak) from this run will be broader than the
first run, because sample is being applied at lower concentration over
a longer period of time.
Section 25
Product Information
Catalog # Description
BioLogic LP Systems
731-8300 Standard BioLogic LP System, 100/120 V, includes
BioLogic LP controller, BioLogic rack, accessory kit with inject
valve (MV-6), proportioning valve/mixer, UV optics, conductivity
cell, tubing and fittings kit, column and conductivity cell holder,
starter kit, instructions
731-8301 Standard BioLogic LP System, 220/240 V
731-8302 BioLogic LP System, With Model 2110 Fraction
Collector,100/120 V, includes standard BioLogic LP system,
SV-3 diverter/bypass valve, system cable 1
731-8303 BioLogic LP System, With Model 2110 Fraction
Collector, 220/240 V
731-8336 BioLogic LP System, With Model 2110 Fraction
Collector and LP Data View Software, 100/120 V, includes
SV-3 diverter/bypass valve system cable 1, 25’ serial cable
731-8337 BioLogic LP System, With Model 2110 Fraction
Collector and LP Data View Software, 220/240 V
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