Bio-Rad BioLogic LP System - Manual (Page 21)

Example LP Data View Chromatogram
Example of chromatogram when running the BioLogic LP starter kit.
Section 24
Advanced Users: Automatic Sample
Injection
If your system is equipped with an SV-5 buffer select valve, you may
use it to inject the sample. This feature is important when you have a large
sample, or wish to do multiple runs automatically. To demonstrate this fea-
ture:
1. Use the syringe to dilute a 2 ml aliquot of sample (as prepared above)
to a total volume of 6 ml; place the diluted sample in a centrifuge tube
or other small container.
2. Attach a short length of 0.8 mm ID tubing to port “C” of the SV-5 buffer
select valve; place the other end of the tube in the container of diluted
sample. Bleed the air from the tubing by selecting buffer “C”, and
running at 1.5 ml/minute until the air is removed from the tubing. Flush
the air from the system. (If installed, use the column bypass valve to
avoid pumping air into the column.)
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