Bio-Rad BioLogic LP System - Manual (Page 6)

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Section 3
Buffer and Sample Preparation
Buffer “A”
1. Empty the contents of the bottle labeled “Buffer A” into a 500 ml
graduated cylinder.
2. Add degassed, deionized water to 500 ml total volume.
3. Place contents in bottle.
4. Stir briefly and gently on stir plate.
5. Label container as buffer “A”. (Pull label off buffer “A” bottle and place
on container.)
Buffer “B”
1. Empty the contents of the bottle labeled buffer “B” into a 500 ml
graduated cylinder.
2. Add degassed deionized water to 500 ml total volume.
3. Place contents in bottle.
4. Stir briefly and gently on stir plate.
5. Label container as buffer “B”. (Pull label off buffer “B” bottle and place
on container.)
Protein Sample
1. Dissolve the anion exchange standards in buffer “A”
2. Use the syringe or a pipet to measure 4 ml of buffer “A” into the bottle
of protein standards.
3. Replace the stopper and shake gently to dissolve.
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