Bio-Rad BioLogic LP System - Manual (Page 5)

Section 1
Introduction
This manual guides you through the installation of your new BioLogic
LP chromatography system. You will perform an actual chromatographic
separation, demonstrating the simplicity and excellent performance of the
BioLogic LP.
Section 2
Starter Kit Components
This starter kit contains:
• One Bio-Scale ™ Mini Macro-Prep ® High Q anion exchange cartridge,
1 ml
• Buffer A, 10x concentrate, 50 ml (when diluted: 25 mM Tris-HCl, pH 8.1)
• Buffer B, 10x concentrate, 50 ml (when diluted: Buffer “A” with 0.5 M
NaCl)
• One vial of anion exchange protein standards—equine myoglobin,
conalbumin, chicken ovalbumin, and soybean trypsin inhibitor. Total
9 mg of protein per vial
• One disposable syringe (for injecting sample)
• Instruction manual
For the first-time user, system setup will require 3 to 4 hours; the
demonstration chromatography run requires approximately 30 minutes.
The starter kit contains enough buffer and sample for two separations.
Other Materials You Will Need:
• Degassed deionized water, 1 L
• One 500 ml graduated cylinder
• Two 500 ml bottles for buffers
• Bottle or drain for waste fluid
• 13 collection tubes for fraction collector
• Scissors or blade to cut tubing
• BioLogic LP fittings kit
1
4006073D.qxp 8/17/2009 7:45 AM Page 1