Shellab BACTRONEZ - Manual (Page 36)

36 | P a g e
OPERATION (CONTINUED)
V ERIFYING AN A NAEROBIC A TMOSPHERE
This procedure verifies that an anaerobic atmosphere has been established prior to placing
anaerobe samples in the workstation.
1. After entering the workspace chamber, open one (1) of the Oxoid brand
anaerobic indicator strip packages.
a. Do not touch the indicator strip. Doing so risks contaminating the
strip and creating a false positive.
b. For the best accuracy, and to lengthen the amount of usable time,
the strip should be left in the packet, partly exposed, to avoid
contamination from surfaces, and to wick oxygen detecting fluid
from bottom of the packet.
c. Bending the edges or partly folding the packet will allow it to stand
upright.
d. Once an indicator strip has dried out, it can no longer indicate the
presence of oxygen.
e. There should be one, opened, moist anaerobic indicator strip in the workspace
chamber at all times while maintaining anaerobic conditions. Place the strip
where it will be clearly visible.
2. If the strip remains white, the chamber atmosphere is free of oxygen and ready to use. Exit
the chamber using the steps described in the Exiting the Chamber procedure on page
37.
3. If the strip turns partly or completely pink, exit the chamber using the Exiting the Chamber
procedure steps to avoid introducing more oxygen into the chamber.
a. Loosen the right arm port door to create a slight leak that aerobic atmosphere
can be expelled through. See Step 11 of the Establish an Anaerobic
Environment procedure on page 33.
b. Flush an additional 50 psi or 30 minutes worth of AMG through the chamber.
Note: The sleeve assemblies can be left on the BACTRONEZ during this second purge.
c. When 30 minutes has elapsed or 50 psi of AMG have been flushed through
the chamber (whichever comes first), re-enter the chamber using correct entry
procedures, and open a second anaerobic indicator strip.
d. If the second anaerobic indicator strip shows less pink than before, repeat
steps b and c to finish purging the chamber.
e. If the second strip turns completely pink or shows the same degree of coloring
as the first, contact Sheldon Technical Support for assistance. See page 6.
Also, see the optional troubleshooting procedure on the next page for steps to
identify possible sources of oxygen in the work station.
Figure 23: Pink indicating
the presence of oxygen in
an office environment