Teledyne 10 - Manual (Page 66)

BioOptix™ 10
Appendix B Fluid Path Diagrams
B-2
While the system is running a fluid-type block:
• The Buffer valve switches between buffers A and B to
produce the correct mix.
• The Sample valve allows only buffer solution to pass.
• If the channel is active, the Channel valve allows the
fluid to be pumped. If the channel is inactive, the valve
prevents fluid from being pumped (Figure B-2).
• The Mode valve is in the Operate position.
• The Effluent valve can direct the fluid to waste or to the
fraction collector. Directing the effluent to waste is
practical when the fluid block is in the experiment for
column equilibration. Collecting the effluent may be
desirable if the fluid may contain something of interest
(e.g. during a column wash).
Figure B-2 Inactive channel
B.2 Load-type Blocks Load blocks apply the sample onto the column. There are two
modes that can be selected in the Method file—Pump or Manual
injection.
Pump injections draw liquid sample from the Sample In port and
routes it to the column (Figure B-3). The Mode valve should be in
the Operate position; the Effluent valve can either collect the
fluid or divert it to the channel’s Waste port.
Figure B-3 Fluid path for Load-type blocks -
Pump injections
Channel
Pump
Column
Sample
Channel
Mode
Effluent
Pump
To Foxy
Sample In
Column