Bio-Rad Mini-PROTEAN® TGX™ Precast Gels for 2-D Electrophoresis - Manual (Page 11)

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2.2 Required Materials
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Mini-PROTEAN ® precast gels
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Mini-PROTEAN ® Tetra cell (or Mini-PROTEAN ® 3 Dodeca ™ , Mini-PROTEAN II or
Mini-PROTEAN 3 cell)
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PowerPac ™ Basic or PowerPac HC power supply (or equivalent); PowerPac HV or
PowerPac Universal required for high-voltage applications (>300 V)
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Sample buffer
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Running buffer (750 ml for 1–2 gels; 1,000 ml for 3–4 gels or when running at
voltages >200 V)
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Opening lever (catalog #456-0000)
2.3 Setting Up and Running Mini-PROTEAN Gels in the
Mini-PROTEAN Tetra Cell
1. Remove the gels from the storage pouch and prepare them for assembly:
a. Remove the comb: Position thumb on the indentation (middle of comb) and remove the comb
by pulling upward in one smooth motion.
b. Remove the tape: Pull gently to remove the green tape from the bottom of the cassette.
If necessary, use the opening key or comb to help remove the tape at the corners.
c. Rinse the wells: Use a syringe, wash bottle, or disposable transfer pipet to rinse the wells with
Remove the comb
Remove the tape
Instruction Manual and Application Guide